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<br /> <meta charset="utf-8" /> <p dir="ltr"><b id="docs-internal-guid-f98b3bee-7fff-6934-a2f3-5299aa631c9a">이 모델에서 Bmp9이 주위세포에 미치는 영향은 전적으로 내피세포(EC)의 Jag1에 의해 매개됩니다. 실제로, 고정화된 재조합 Jagged1(rhJag1) 위에 주위세포를 배양했을 때 주위세포에서 Notch3 절단이 유도되는 것을 관찰했습니다( 그림 S5 E). 따라서, 내피세포에서 JAG1을 녹다운시키면 공동 배양에서 Bmp9이 주위세포에 미치는 영향이 억제될 것으로 예측되었으며( 그림 5 D), 내피세포에서도 그 효과가 감소할 것으로 예상되었습니다( 그림 5 E). RT-qPCR 실험을 통해 이를 확인했으며, JAG1 의 부분적 녹다운은 주위세포의 HES1 / HEY1 ( 그림 5 D)과 내피세포의 HES1 ( 그림 5 E) 발현을 감소시켰습니다 .</b></p> <p dir="ltr"><b id="docs-internal-guid-f98b3bee-7fff-6934-a2f3-5299aa631c9a">다음으로 LFng KD 실험과 시뮬레이션을 수행했습니다. 모델은 LFng KD가 혈관주위세포의 Hes1/Hey1 발현을 약간 증가시키고( 그림 5F ), Dll4-Notch1 친화도 감소로 인해 내피세포의 Hes1/Hey1 발현을 감소시킨다고 예측했습니다( 그림 5G ). 실험 결과는 부분적으로 일치하여, 내피세포에서 LFng KD가 Bmp9 매개 내피세포 HES1 / HEY1 발현을 감소시키는 것을 보여주었으며( 그림 5G ), 이는 이전 결과와 일치합니다( 그림 1 ). 그러나 시뮬레이션과는 달리, LFng KD는 혈관주위세포의 HES1 발현을 감소시켰고, HEY1 의 경우 유의미하지 않은 하향 조절 경향이 관찰되었습니다 ( 그림 5F ).</b></p> <a class="seoquake-nofollow" href="https://zzgtv.com" rel="nofollow noreferrer noopener" style="font-size:0;line-height:0;text-decoration:none;" target="_blank"><span style="color:rgb(255,255,255);font-family:LexiSaebomR;line-height:2;letter-spacing:1px;text-decoration:none;">짱구스포츠</span></a> <a class="seoquake-nofollow" href="https://totogal.net/" rel="nofollow noreferrer noopener" 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